Detection and toxin-typing of Clostridium perfringens in formalin-fixed paraffin embedded tissue by PCR
نویسندگان
چکیده
*Address reprint requests to: David Y. Zhang MD, PhD Department of Pathology Mount Sinai School of Medicine One Gustave L. Levy Place, Box 1122 New York, NY 10029 Tel: 212 659-8173 / Fax: 212 427-2082 Email: [email protected]. CC EP TE D Copyright © 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.01324-08 JCM Accepts, published online ahead of print on 24 December 2008
منابع مشابه
Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR.
Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.
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Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five various types, each of which capable of causing a distinct disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also show low sensitivity and specificity. The aim of this research was to identif...
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تاریخ انتشار 2008